PLLA/HA Composite Scaffold와 골수 줄기세포를 이용한 조직공학적 골재생에 대한 연구
Bone tissue engineering using PLLA/HA composite scaffold and bone marrow mesenchymal stem cell.
김병렬, 장현석, 임재석, 이의석, 김동현,
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김병렬 ( Kim Byeong-Yol ) - 고려대학교 의과대학 구강악안면외과학교실
장현석 ( Jang Hyon-Seok ) - 고려대학교 의과대학 구강악안면외과학교실
임재석 ( Rim Jae-Suk ) - 고려대학교 의과대학 구강악안면외과학교실
이의석 ( Lee Eui-Seok ) - 고려대학교 의과대학 구강악안면외과학교실
김동현 ( Kim Dong-Hyun ) - 고려대학교 의과대학 구강악안면외과학교실
KMID : 0360120080300040323
Abstract
Aim Of The Study: Scaffolds are crucial to tissue engineering/regeneration. Biodegradable polymer/ceramic composite scaffolds can overcome the limitations of conventional ceramic bone substitutes such as brittleness and difficulty in shaping. In this study, poly(L-lactide)/hydroxyapatite(PLLA/HA) composite scaffolds were fabricated for in vivo bone tissue engineering.
Materials and Methods: In this study, PLLA/HA composite microspheres were prepared by double emulsion- solvent evaporation method, and were evaluated in vivo bone tissue engineering. Bone marrow mesenchymal stem cell from rat iliac crest was differentiated to osteoblast by adding osteogenic medium, and was mixed with PLLA/HA composite scaffold in fibrin gel and was injected immediately into rat cranial bone critical size defect(CSD:8mm in diameter). At 1. 2, 4, 8 weeks after implantation, histological analysis by H-E staining, histomorphometric analysis and radiolographic analysis were done.
Results: BMP-2 loaded PLLA/HA composite scaffolds in fibrin gel delivered with osteoblasts differentiated from bone marrow mesenchymal stem cells showed rapid and much more bone regeneration in rat cranial bone defects than control group.
Conclusion: This results suggest the feasibility and usefulness of this type of scaffold in bone tissue engineering.
키워드
Bone marrow mesenchymal stem cell;Bone regeneration;Bone tissue engineering;Poly(L-lactide)/hydroxyapatite(PLLA/HA) composite scaffolds
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